Introduction to Sequence Analysis (BCMA III)7.5 ECTS
(englische Bezeichnung: Einführung in die Sequenzanalyse)
(Prüfungsordnungsmodul: BCMA III: Pflanzenbiotechnologie)

Lectures:

• • Introduction to Sequence Analysis
    (Übung, 8 SWS, Anwesenheitspflicht, Uwe Sonnewald et al., Zeit und Raum n.V.)

Empfohlene Voraussetzungen:

keine

Inhalt:

• Introduction to sequence analysis and sequence formats.

• Primer design, sequence alignments and sequence assembly.

• Sequence databases, similarity search (BLAST) and DNA barcoding.

• Genomic and genetic structures.

• Gene analysis and alternative splicing.

• Regulation of gene expression.

• Promoter analysis and prediction of TBFs and microRNA targets.

• Genetic markers (SNPs, microsatellites and INDELs).

• Next Generation Sequencing (NGS) dataprocessing.

• Genome mapping and annotation.

• Transcriptome analysis methods (Microarray and RNA-seq).

• Epigenetic local and high-throughput analysis.

• Molecular diagnosis and personalized medicine.

• SNPs analysis for disease human prediction.

• Mutagenesis and directed evolution.

• Strategical workflow and establishment of analytical pipelines.
  

Lernziele und Kompetenzen:

Students able to:

• use databases, online tools and software for sequence analysis.

• practice with similarity search of sequences (BLAST).

• predict and categorize sequence features as gene-structures, reading frames, active sites, post-translational modification sites, distribution of exons and introns, and regulatory elements.

• discover genetic markers by identification of sequence variations as SNPs, INDELs or microsatellites.

• predict different levels of mRNA and protein structures.

• analyze and compute Next Generation Sequencing datasets.

• relate genomic and transcriptomic data with phenotype.

• generate analytical workflows combining different applications and formats.

• practice with different sequence datasets and develop an own hypothetical project of sequence analysis.

• prepare a presentation and a written scientific report describing and discussing the results obtained in the practical project; synthesizing and contrasting the information of scientific publications with own data.

Literatur:

keine

Verwendbarkeit des Moduls / Einpassung in den Musterstudienplan:

1. Zell- und Molekularbiologie (Master of Science)
   (Po-Vers. 2015w | NatFak | Zell- und Molekularbiologie (Master of Science) | Mastermodule | Biologische Mastermodule | BCMA III: Introduction to Sequence Analysis)

Studien-/Prüfungsleistungen:

BCMA III: Introduction to Sequence Analysis (Prüfungsnummer: 22802)

Prüfungsleistung, mehrteilige Prüfung, benotet, 7.5 ECTS

Anteil an der Berechnung der Modulnote: 100.0 %

weitere Erläuterungen:
PL: oral examination (30 min.) PL: Protokol (approx. 3-5 pages)

Erstablegung: SS 2019

1. Prüfer:

Uwe Sonnewald