Das Projekt beschäftigt sich mit den regulatorisch aktiven Proteinen IE86 und pUL69 des humanen Cytomegalovirus, die eine essentielle Funktion im viralen Replikationszyklus haben und von daher neue attraktive Zielmoleküle für antivirale Therapieansätze darstellen. Während IE86 durch Interaktion mit weiteren, zellulären Proteinen die Genexpression aktiviert, scheint UL69 die Transkriptionselongation sowie den RNA-Export zu beeinflussen.Publikationen(ab 2000):
Bedard, J., May, S., L`Heureux, L, Stamminger, T., Copsey, A., Drach, J., Huffman, J., Chan, L., Jin, H., and Rando, R. (2000). Antiviral properties of a series of 1,6-naphthyridine and dihydroisoquinoline derivatives exhibiting potent activitiy against human cytomegalovirus. Antimicrobial Agents and Chemotherapy, 44, 929-937.
Hofmann, H., Flöß, S., and Stamminger, T. (2000). Covalent modification of the transactivator protein IE2-p86 of human cytomegalovirus by conjugation to the ubiquitin-homologous proteins SUMO-1 and hSMT3b. J. Virol., 74, 2510-2524.
Marschall, M., Freitag, M., Weiler, S., Sorg, G., and Stamminger, T. (2000). Recombinant GFP-expressing human cytomegalovirus as a tool for screening of antiviral agents. Antimicrobial Agents and Chemotherapy, 44, 1588-1597.
Winkler, M., aus dem Siepen, T., and Stamminger, T. (2000). Functional interaction between the pleiotropic transactivator pUL69 of human cytomegalovirus and the human homolog of the yeast chromatin regulatory protein SPT6. J. Virol., 74, 8053-8064.
Marschall, M., Stein-Gerlach, M., Freitag, M., Kupfer, R., van den Bogaard, M., and Stamminger, T. (2001). Inhibitors of human cytomegalovirus replication drastically reduce the activity of the viral protein kinase pUL97. J. Gen. Virol., 82, 1439-1450.
Lischka, P., Rosorius, O., Trommer, E., and Stamminger, T. (2001). A novel transferable nuclear export signal mediates CRM1 independent nucleo-cytoplasmic shuttling of the human cytomegalovirus transactivator protein pUL69. EMBO J., 20, 7271-7283.
Kronschnabl, M., Marschall, M. and Stamminger, T. (2002). Efficient and tightly regulated expression systems for the human cytomegalovirus major transactivator protein IE2p86 in permissive cells. Virus Res., 83, 89-102.
Efferth, T., Marschall, M., Wang, X., Huong, S.-M., Hauber, I., Olbrich, A., Kronschnabl, M., Stamminger, T., and Huang, E.-S. (2002). Antiviral Activity of artesunate towards wild-type, recombinant and ganciclovir-resistant human cytomegaloviruses. J. Mol. Med., 80, 233-242.
Marschall, M., Stein-Gerlach, M., Freitag, M., Kupfer, R., van den Bogaard, M., and Stamminger, T. (2002) Direct targeting of human cytomegalovirus protein kinase pUL97 by kinase inhibitors is a novel principle for antiviral therapy. J. Gen. Virol., 83, 1013-1023.
Stamminger, T., Gstaiger, M., Weinzierl, K., Lorz, K., Winkler, M., and Schaffner, W. (2002). Open reading frame UL26 of human cytomegalovirus encodes a novel tegument protein that contains a strong transcriptional activation domain. J. Virol., 76, 4386-4847.
Hofmann, H., Sindre, S., and Stamminger, T. (2002). Functional interaction between the pp71 protein of human cytomegalovirus and the PML-interacting protein human Daxx. J. Virol., 76, 5769-5783.
Rehberg, S., Lischka, P., Glaser, G., Stamminger, T., Wegner, M., and Rosorius, O. (2002). SOX10 is an active nucleo-cytoplasmic shuttle protein and shuttling is crucial for SOX10-mediated transactivation. Mol. Cell. Biol., 22, 5826-5834.
Kronschnabl, M., and Stamminger, T. (2002). Synergistic induction of ICAM-1 by the human cytomegalovirus transactivator IE2p86 and pp71 is mediated via a Sp1 binding site. J.Gen. Virol., in press.